Development of Ogura CMS Fertility-Restored Interspecific Hybrids for Use in Cytoplasm Replacement of Golden-Heart Materials in Brassica rapa

Ogura cytoplasmic male sterility (CMS) is one of the important methods for hybrid seed production in cruciferous crops. The lack a restorer fertility gene (Rfo) Brassica rapa L. restricts development and utilization its germplasm resources. In this research, napus with Rfo was used to restore CMS B. golden heart trait. Through distant cross two four napus, six interspecific combinations received F1 seeds. were different receiving. By morphological observation molecular marker-assisted selection (MAS), F1, individuals containing all appeared fertile, while those without it remained male-sterile. pollen viability fertile measured, lines (40.68–80.49%). Three (containing both GOLDEN genes) highest vitality (≥60%) backcrossed rapa, resulting total 800 plants. Based on MAS, 144 plants but no screened (18%). Moreover, through investigation, individual normal cytoplasm, stable restoring Rfo, gene, characteristics similar obtained. These results increased diversity provided new method